ANTITERMINATION CONTROL OF POL II TRANSCRIPTION

  • Kellems, Rodney E, (PI)

Project: Research projectResearch Project

Description

Recent evidence indicates that transcription arrest or termination sites
are situated near the 5' ends of many eukaryotic cellular and viral
genes and that transcription through these sites is subject to metabolic
or developmental control. The number and diversity of examples suggest
that antitermination of transcription is a prevalent mechanism for
controlling gene expression in eukaryotic cells. To understand this
important gene regulatory process we have chosen to study the expression
of the murine and human adenosine deaminase (ADA) genes. Evidence from
my laboratory suggests that cell specific regulation of ADA gene
expression results from antitermination mechanisms that override or
disrupt a transcription termination signal that resides within the 3'
end of exon 1. Research proposed here will determine the sequence and
factor requirements for the exon 1 transcription termination event and
the means by which it is developmentally controlled in a cell specific
manner. As a result of proposed experiments we expect to determine: 1)
The sequence requirements for transcription termination in exon 1 of the
murine and human ADA genes, 2) The role of antitermination mechanisms in
the regulation of ADA gene expression, and 3) The protein components
affecting transcription termination and antitermination. The results of
these experiments should allow us to distinguish among three general
models for the control of transcription termination: 1) Modification of
the RNA polymerase complex, 2) Modification of RNA secondary structure,
and 3) Steric interference of transcription elongation by direct DNA
binding. The research proposed here should lead to a better
understanding of the mechanism of exon 1 transcription termination and
the role of antitermination in the regulation of gene expression in
mammalian cells.
StatusFinished
Effective start/end date3/1/822/29/96

Funding

  • National Institutes of Health: $161,044.00
  • National Institutes of Health
  • National Institutes of Health: $90,715.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Tetrahydrofolate Dehydrogenase
Genes
Exons
Consensus Sequence
Adenosine Deaminase
Gene Expression
Gene Expression Regulation
RNA Polymerase II
Genetic Terminator Regions
Research
Eukaryotic Cells
DNA-Directed RNA Polymerases
Regulator Genes
beta-Globins
Cricetinae
Vertebrates
Chickens
RNA
Clone Cells
Genome

Keywords

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)