AXONAL REGENERATION AND NERVE GROWTH FACTOR

  • Hulsebosch, Claire E, (PI)

Project: Research project

Description

This proposal is designed to begin to study the parameters of axonal
sprouting induced by Nerve Growth Factor (NGF) and by spinal cord
denervation. The methods will be ultrastructural quantitation of the
myelinated and unmyelinated axons of appropriate nerves and roots, light
microscopic quantitation of neurons and autoradiography. Preliminary data
indicate that endogenous NGF is implicated in the sprouting of central
processes of dorsal root ganglion neurons after spinal cord denervation at
birth. Other preliminary evidence indicates that a small population of
dorsal root ganglion neurons are labeled with radiolabeled-NGF after spinal
cord injury suggesting that a specific group of neurons may sequester NGF
after injury. Consequently the obectives of this proposal are: 1) to
quantify the effects of nerve growth factor in the uninjured mammal by
examination of specific neural pathways in rats treated with NGF or the
antibody to NGF, 2) to quantify the involvements of NGF in the sprouting
response of dorsal root ganglion neurons after spinal denervation by
administering NGF or its antibody to rats with spinal damage, 3) to
investigate the dorsal root ganglion neuron population that binds
radiolabeled NGF after spinal cord denervation. Given that the amount of sprouting may be related to functional recovery
and that endogenous NGF would seem to be implicated in the response to
spinal cord denervation, the establishment of these parameters may lead to
futher studies designed to test the clinical applicability of these
manipulations.
StatusFinished
Effective start/end date7/1/8412/31/87

Funding

  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Nerve Growth Factor
Regeneration
Denervation
Neurons
Spinal Ganglia
Spinal Cord
Neurotrophin 3
Neural Pathways
Wounds and Injuries
Autoradiography
Ganglia
Population
Axons
Mammals
Antibodies

Keywords

  • Medicine(all)
  • Neuroscience(all)