BIOSENSOR TEST ANESTHETIC EFFECTS ON CARDIAC MEMBRANES

  • Hixson, James E, (PI)
  • Schultz, Jim (PI)
  • Driscoll, Donna (PI)
  • Chanh, Tran (PI)
  • Henkel, Richard (PI)
  • Taraporewala, Irach (PI)
  • Centner, Peter (PI)
  • Lewis, Douglas (PI)
  • Huot, Rachel (PI)
  • Treichel, Robin (PI)
  • Kushwaha, Rampratap (PI)
  • Purdy, Robert (PI)
  • Sureau, Camille (PI)
  • Minoo, Parviz (PI)
  • Coelho, Anthony (PI)
  • Beames, Burton (PI)
  • Attanasio, Roberta (PI)
  • Warren, Ronald (PI)
  • Kanda, Patrick (PI)
  • Somawardhana, C. (PI)
  • Hilliard, Julia (PI)
  • Konigsberg, L. (PI)
  • McGill, Henry (PI)
  • Downer, Nancy (PI)
  • Lin, Alan (PI)
  • Rainwater, David (PI)
  • Dyke, Bennett (PI)
  • Kammerer, Candace (PI)
  • Blangero, John (PI)
  • Rice, Karen (PI)
  • Pastorcic, Martine (PI)
  • Mark Sharp, R. (PI)
  • Blangero, John (PI)

Project: Research projectBiomedical Research Support Grants, Small Research Grants

Description

The proposed study will test the feasibility of using a capacitive
biosensor based on an interdigitated electrode array to monitor the
interaction of volatile anesthetics with cardiac membrane proteins that
play a role in calcium homeostasis and are thought to be sties at which the
volatile anesthetics act to depress cardiac contractility. A biosensor can
be defined as a system in which a biological recognition system is coupled
with a transducing device to give an electrical output that is directly
usable for either measurement or control purposes. If it can be shown that
such biosensors are sufficiently sensitive and that the outputs can be
related to specific bimolecular processes, then this technology has
enormous potential for testing pharmacologic activity and the interaction
of agents that ct on membrane receptors. The voltage-dependent Ca2+ channel and Ca2+/Mg2+-ATPase from cardiac muscle
will be reconstituted into membrane-like structures at the surface of a
planar capacitive biosensor. The capacitance response of the system to the
application of agonists, antagonists, halothane, and to various
combinations of these agents will be measured. Membrane assemblies
prepared by the same technique will be studied by Fourier transform
infrared (FTIR) spectroscopy through use of attenuated total reflectance
(ATR) techniques for obtaining spectral data from surface films.
Biomembranes incorporating membrane proteins as well as assemblies formed
from phospholipids alone will be observed by FTIR in order to identify the
sites at which the agents bind and their effects on protein or lipid
structure. Structural information obtained by FTIR will be correlated with
electrical response from the biosensor to determine whether this type of
biosensor can contribute to mechanistic studies and/or rapid monitoring of
drug and anesthetic interactions.
StatusFinished
Effective start/end date4/1/799/29/93

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Biosensors
Anesthetics
Membranes
LDL Receptors
Apolipoproteins B
Membrane Proteins
Liver
Ca(2+) Mg(2+)-ATPase
Genes
Halothane
Apoprotein(a)
Phospholipids
Capacitance
Spectroscopy
Calcium
Protein Isoforms
Electrodes
Monitoring
Testing
Electric potential

Keywords

  • Medicine(all)