HORMONAL REGULATION OF ISOLATED GASTRIC MUCOSAL CELLS

  • Rosenfeld, Gary C, (PI)

Project: Research projectResearch Project

Description

The objective of this research is to understand how secretagogues and
secretory inhibitors regulate and coordinate the secretion and production
of hydrogen ions from parietal cells isolated from the rat gastric mucosa.
Enriched and purified parietal cells will be used to study secretagogue
stimulation of acid secretion as well as related biochemical and
cytoskeletal modifications associated with receptor activation. We plan to
detect and more precisely characterize several cell membrane receptors
using techniques of radiolabeled ligand binding. We also plan to examine
biochemically the role of cyclic nucleotide metabolism, calmodulin, and
cytoskeletal proteins in cell regulation by secretagogues. We will test
our hypothesis that hydrogen ion production stimulated by acetylcholine and
histamine is due to an interaction between calcium regulated intracellular
events and accumulated cyclic AMP. Additionally, immunofluorescent
cytochemical techniques will be used to localize components of both the
cyclic nucleotide metabolic system and cytoskeletal structure which may be
specifically involved in parietal cell function. This research should lead
to a better understanding of the regulation of gastric acid secretion.
Also, this research should identify alternative loci for possible
pharmacological intervention necessary to control hydrogen ion secretion
and peptic disease.
StatusFinished
Effective start/end date5/1/834/30/86

Funding

  • National Institutes of Health

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Stomach
Protons
Research
Cyclic Nucleotides
Gastric Acid
Calmodulin
Gastric Mucosa
Cyclic AMP
Acetylcholine
Digestion
Nucleotides
Cell Membrane
Ligands
Calcium
Acids
Proteins

Keywords

  • Medicine(all)