MECHANISM OF ACTION OF NEUROPEPTIDES IN ENDOCRINE CELLS

  • Schonbrunn, Agnes, (PI)

Project: Research projectResearch Project

Description

The objective of this rsearch plan is to investigate the mechanism by which
neuropeptides regulate hormone secretion and synthesis by target cells.
The studies will utilize two clonal cell lines derived from anterior
pituitary tissue and one clonal cell line derived from the endocrine
pancreas. These cell lines have retained many of the differentiated
functions of the gland of origin and possess several unique properties
which readily permit studies that would be difficult or impossible to
perform in other systems. Aspects of the mechanism of action of two
neuropeptides will be examined: somatostatin and bombesin. Five specific
areas will be investigated. 1. The regulation of hormone secretion and
synthesis by target cells will be characterized for each peptide. The
validity of the cell lines as model systems will be tested by comparing the
biological responses of each of the cell strains with the response of the
target gland In Vivo and in primary culture; 2. Specific receptors for
each peptide will be characterized both by binding studies with
radiolabeled peptide analogs and biochemically. 3. The regulation of
receptors by homologous and heterologous ligands will be examined and
related to changes in target cell responsiveness; 4. The internalization
and processing in receptor regulation; 5. The role of cyclic nucleotides
and protein phosphorylation in the mechanism by which these neuropeptides
regulate hormone secretion will be determined. The methodology will
involve primarily biochemical and immunological procedures. These studies
will provide progress towards the long-term objectives of this laboratory:
namely understanding, at a molecular level, the biochemical steps involved
in the action of neuropeptides on endocrine glands from the initial
interaction with target cell receptors to the final cellular response.
StatusFinished
Effective start/end date4/1/8311/30/86

Funding

  • National Institutes of Health

Fingerprint

Endocrine Cells
Neuropeptides
Cell Line
Hormones
Endocrine Glands
Bombesin
Peptides
Peptide Receptors
Somatostatin
Phosphorylation
Ligands
Proteins

Keywords

  • Medicine(all)