PLATELET ACTIVATION AND MICROFILAMENT ORGANIZATION

  • Rosenfeld, Gary C, (PI)

Project: Research projectResearch Project

Description

The objective of this research proposal is to understand the role of
vinculin in platelet activation and microfilament organization. Our
working hypothesis is that vinculin exist in several forms and following
platelet activation one or more of these forms is modified to allow its
interaction with actin, other actin associated proteins, or the plasma
membrane. To test this hypothesis we plan to isolate cytoskeletal and
membrane fractions from unactivated and activated platelets and probe for
vinculin's distribution and localization by immunoblot analysis. We also
will study the in vitro interaction of the soluble platelet vinculin we
have purified with actin and actin binding proteins using viscometric,
fluorometric, sedimentation, affinity matrix and gel overlay analyses.
Further, we will study the effects of calcium and phosphorylation on the
distribution and activity of vinculin in unactivated, activated, adherent,
and aggregated platelets. These studies should provide fundamental
information on the role of vinculin in cytoskeletal organization and also
in platelet activation.
StatusFinished
Effective start/end date7/1/8411/30/87

Funding

  • National Institutes of Health
  • National Institutes of Health

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Vinculin
Platelet Activation
Actin Cytoskeleton
Actins
Blood Platelets
Microfilament Proteins
Research Design
Gels
Phosphorylation
Calcium
Proteins

Keywords

  • Medicine(all)