Association of single nucleotide autophagy-related protein 5 gene polymorphism rs2245214 with susceptibility to non–small cell lung cancer

Mohsen Nikseresht, Maryam Shahverdi, Mehdi Dehghani, Hassan Abidi, Reza Mahmoudi, Ghasem Ghalamfarsa, Leila Manzouri, Saeid Ghavami

Research output: Contribution to journalArticle

Abstract

Introduction: Autophagy is a mechanism that is involved in the regulation of cellular life, apoptosis, and stemness while its intervening genes play important functions in various cancers including lung cancer. ATG5 is one of the key genes for the regulation of the autophagy pathway. In this study, our team has investigated the potential relationship between ATG5 gene polymorphism rs2245214 with non–small cell lung cancer (NSCLC) in a subpopulation of patients from southern Iran. In this study, 34 patients with NSCLC (20 males and 14 females [mean age: 12.86 ± 60.47 years]) and 50 healthy subjects (30 males and 20 females [mean age: 13.09 ± 56.62 years]) were studied in terms of the genotype of the ATG5 gene. We used restriction fragment length polymorphism and analyzed the results using SPSS software (v.23). The results revealed that subjects harboring the guanine/cytosine (GC) genotype of the rs2245214 ATG5 gene polymorphism had suffered less from NSCLC, whereas the prevalence of the C-allele of this polymorphism was significantly higher in patients with NSCLC (P < 0.05). On the basis of the results of logistic regression, the presence of this C-allele may predict the risk of lung cancer (P value = 0.011; OR, 3.52; 95% CI, 1.33-9.26). This study concludes that the C-allele of the rs2245214 ATG5 gene polymorphism is associated with increased susceptibility to NSCLC, whereas the GC genotype of this polymorphism is associated with decreased risk and might therefore have a protective role in the development of NSCLC.

LanguageEnglish
Pages1924-1931
Number of pages8
JournalJournal of cellular biochemistry
Volume120
Issue number2
DOIs
StatePublished - Feb 1 2019
Externally publishedYes

Fingerprint

Polymorphism
Non-Small Cell Lung Carcinoma
Nucleotides
Genes
Cells
Lung Neoplasms
Proteins
Cytosine
Alleles
Genotype
Autophagy
Guanine
Iran
Restriction Fragment Length Polymorphisms
Autophagy-Related Protein 5
Logistics
Healthy Volunteers
Software
Logistic Models
Apoptosis

Keywords

  • ATG5
  • autophagy
  • non–small cell lung cancer (NSCLC)
  • polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Association of single nucleotide autophagy-related protein 5 gene polymorphism rs2245214 with susceptibility to non–small cell lung cancer. / Nikseresht, Mohsen; Shahverdi, Maryam; Dehghani, Mehdi; Abidi, Hassan; Mahmoudi, Reza; Ghalamfarsa, Ghasem; Manzouri, Leila; Ghavami, Saeid.

In: Journal of cellular biochemistry, Vol. 120, No. 2, 01.02.2019, p. 1924-1931.

Research output: Contribution to journalArticle

Nikseresht, M, Shahverdi, M, Dehghani, M, Abidi, H, Mahmoudi, R, Ghalamfarsa, G, Manzouri, L & Ghavami, S 2019, 'Association of single nucleotide autophagy-related protein 5 gene polymorphism rs2245214 with susceptibility to non–small cell lung cancer', Journal of cellular biochemistry, vol. 120, no. 2, pp. 1924-1931. https://doi.org/10.1002/jcb.27467
Nikseresht, Mohsen ; Shahverdi, Maryam ; Dehghani, Mehdi ; Abidi, Hassan ; Mahmoudi, Reza ; Ghalamfarsa, Ghasem ; Manzouri, Leila ; Ghavami, Saeid. / Association of single nucleotide autophagy-related protein 5 gene polymorphism rs2245214 with susceptibility to non–small cell lung cancer. In: Journal of cellular biochemistry. 2019 ; Vol. 120, No. 2. pp. 1924-1931.
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AU - Abidi, Hassan

AU - Mahmoudi, Reza

AU - Ghalamfarsa, Ghasem

AU - Manzouri, Leila

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