Biomarkers for Microvascular Proteins Detection: Blood–Brain Barrier Injury and Damage Measurement

Pavani Sayana, Jean Pierre Oses, Tatiana Barichello, Vijayasree V. Giridharan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Blood–brain barrier (BBB) is a highly selective semipermeable membrane with tight junctions formed from closely wedged epithelial cells and is the principal regulator of exchange of materials between the blood and the brain, simultaneously protecting it from the systemic insults. Any impairment in the functional coordinated effort or formation of the components of the BBB neurovascular bundle may lead to neurological disturbances from entry of extraneous elements. The BBB disruption causes an imbalance in tight junction proteins, adherens junction proteins, matrix metalloproteinases, cell adhesion molecules, and a myriad of neuroinflammatory proteins. Therefore, the modalities, which can enable the accurate detection of BBB breach in advance via protein biomarker assay to underpin targeted medicines, are of growing priority. In this chapter, we will summarize the BBB pathology measurement methods used in the detection of microvascular protein biomarkers such as Western blot, immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), and real-time polymerase chain reaction (RT-PCR). In addition, we also emphasize on the BBB genomics and the method used to detect functionally related BBB genes in preclinical model.

LanguageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages343-363
Number of pages21
DOIs
StatePublished - Jan 1 2019

Publication series

NameNeuromethods
Volume142
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Fingerprint

Biomarkers
Blood-Brain Barrier
Brain Injuries
Proteins
Assays
Tight Junction Proteins
Adherens Junctions
Immunosorbents
Tight Junctions
Polymerase chain reaction
Cell Adhesion Molecules
Pathology
Genomics
Matrix Metalloproteinases
Medicine
Real-Time Polymerase Chain Reaction
Brain
Blood
Genes
Western Blotting

Keywords

  • Blood–brain barrier
  • Brain microvasculature
  • Elisa and RT-PCR
  • Immunohistochemistry
  • Tight junction proteins
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

Sayana, P., Oses, J. P., Barichello, T., & Giridharan, V. V. (2019). Biomarkers for Microvascular Proteins Detection: Blood–Brain Barrier Injury and Damage Measurement. In Neuromethods (pp. 343-363). (Neuromethods; Vol. 142). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8946-1_20

Biomarkers for Microvascular Proteins Detection : Blood–Brain Barrier Injury and Damage Measurement. / Sayana, Pavani; Oses, Jean Pierre; Barichello, Tatiana; Giridharan, Vijayasree V.

Neuromethods. Humana Press Inc., 2019. p. 343-363 (Neuromethods; Vol. 142).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sayana, P, Oses, JP, Barichello, T & Giridharan, VV 2019, Biomarkers for Microvascular Proteins Detection: Blood–Brain Barrier Injury and Damage Measurement. in Neuromethods. Neuromethods, vol. 142, Humana Press Inc., pp. 343-363. https://doi.org/10.1007/978-1-4939-8946-1_20
Sayana P, Oses JP, Barichello T, Giridharan VV. Biomarkers for Microvascular Proteins Detection: Blood–Brain Barrier Injury and Damage Measurement. In Neuromethods. Humana Press Inc. 2019. p. 343-363. (Neuromethods). https://doi.org/10.1007/978-1-4939-8946-1_20
Sayana, Pavani ; Oses, Jean Pierre ; Barichello, Tatiana ; Giridharan, Vijayasree V. / Biomarkers for Microvascular Proteins Detection : Blood–Brain Barrier Injury and Damage Measurement. Neuromethods. Humana Press Inc., 2019. pp. 343-363 (Neuromethods).
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