Osteoclast differentiation assay

Jingxuan Yang, Xiaohong Bi, Min Li

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Osteoclasts are highly specialized multinucleated cells derived from the monocyte/macrophage hematopoietic lineage that are uniquely capable of adhering to bone matrix and resorbing bone. The tartrate-resistant acid phosphatase (TRAP) assay is the most common method to detect osteoclasts population in vitro. Here we described a general protocol of inducing osteoclast differentiation from the murine macrophage cell line, RAW264.7, and identification of osteoclasts with the classical TRAP assay.

LanguageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages143-148
Number of pages6
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Fingerprint

Osteoclasts
Osteoblasts
Bone and Bones
Macrophages
Bone Matrix
Cell Line
Population
Tartrate-Resistant Acid Phosphatase

Keywords

  • Bone
  • MCSF
  • Osteoblasts
  • RANK
  • RANKL
  • RAW264.7
  • TRAP staining

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yang, J., Bi, X., & Li, M. (2019). Osteoclast differentiation assay. In Methods in Molecular Biology (pp. 143-148). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8879-2_12

Osteoclast differentiation assay. / Yang, Jingxuan; Bi, Xiaohong; Li, Min.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 143-148 (Methods in Molecular Biology; Vol. 1882).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yang, J, Bi, X & Li, M 2019, Osteoclast differentiation assay. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1882, Humana Press Inc., pp. 143-148. https://doi.org/10.1007/978-1-4939-8879-2_12
Yang J, Bi X, Li M. Osteoclast differentiation assay. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 143-148. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8879-2_12
Yang, Jingxuan ; Bi, Xiaohong ; Li, Min. / Osteoclast differentiation assay. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 143-148 (Methods in Molecular Biology).
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