Oxidative stress in the choroid plexus contributes to blood–cerebrospinal fluid barrier disruption during sepsis development

Mariana Pereira Goldim, Lucineia Gainski Danielski, Judite Filgueiras Rodrigues, Larissa Joaquim, Leandro Garbossa, Aloir Neri de Oliveira Junior, Kiuanne Lino Lobo Metzker, Amanda Della Giustina, Taise Cardoso, Tatiana Barichello, Fabricia Petronilho

Research output: Contribution to journalArticle

Abstract

Background: The choroid plexus (CP), main component of blood–cerebrospinal fluid barrier (BCSFB), protects the brain from peripheral inflammation similar to the blood–brain barrier. Thus, CP is considered a critical target site of oxidative damage, which in sepsis oxidative stress is likely to be a major step in the development of brain damage. Functional alterations in CP may be associated with sepsis-induced brain injury. However, there is no description on the mechanisms associated with BCSFB disruption during sepsis development. Materials and methods: To test this hypothesis, we examined time-dependent oxidative stress markers in CP and permeability of BCSFB in rats submitted to polymicrobial sepsis by cecal ligation and puncture (CLP) or sham surgery (control). We assessed albumin cerebrospinal fluid/plasma concentration quotient (Qalb), an index of BCSFB dysfunction and in CP samples, the oxidative damage in lipids, proteins, antioxidant enzymes and nitrite/nitrate (N/N) concentration in 12, 24 and 48 h after CLP. Results: The increase of BCSFB permeability is time-related to the increase of N/N concentration, oxidative damage to lipid and proteins, and decrease of antioxidant enzyme superoxide dismutase activity at 12 h in the CP; and decrease of catalase activity in 12 and 24 h. Conclusions: In experimental sepsis the BCSFB dysfunction occurs and oxidative stress seems to be a major step in this dysfunction.

LanguageEnglish
Pages19-24
Number of pages6
JournalMicrovascular Research
Volume123
DOIs
StatePublished - May 1 2019

Fingerprint

Cerebrospinal fluid
Choroid Plexus
Oxidative stress
Cerebrospinal Fluid
Sepsis
Oxidative Stress
Blood
Brain
Nitrites
Punctures
Nitrates
Ligation
Permeability
Antioxidants
Lipids
Encephalitis
Enzymes
Blood-Brain Barrier
Catalase
Brain Injuries

Keywords

  • Choroid plexus
  • Neuroinflammation
  • Oxidative stress
  • Sepsis

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Goldim, M. P., Danielski, L. G., Rodrigues, J. F., Joaquim, L., Garbossa, L., de Oliveira Junior, A. N., ... Petronilho, F. (2019). Oxidative stress in the choroid plexus contributes to blood–cerebrospinal fluid barrier disruption during sepsis development. Microvascular Research, 123, 19-24. https://doi.org/10.1016/j.mvr.2018.12.001

Oxidative stress in the choroid plexus contributes to blood–cerebrospinal fluid barrier disruption during sepsis development. / Goldim, Mariana Pereira; Danielski, Lucineia Gainski; Rodrigues, Judite Filgueiras; Joaquim, Larissa; Garbossa, Leandro; de Oliveira Junior, Aloir Neri; Metzker, Kiuanne Lino Lobo; Giustina, Amanda Della; Cardoso, Taise; Barichello, Tatiana; Petronilho, Fabricia.

In: Microvascular Research, Vol. 123, 01.05.2019, p. 19-24.

Research output: Contribution to journalArticle

Goldim, MP, Danielski, LG, Rodrigues, JF, Joaquim, L, Garbossa, L, de Oliveira Junior, AN, Metzker, KLL, Giustina, AD, Cardoso, T, Barichello, T & Petronilho, F 2019, 'Oxidative stress in the choroid plexus contributes to blood–cerebrospinal fluid barrier disruption during sepsis development', Microvascular Research, vol. 123, pp. 19-24. https://doi.org/10.1016/j.mvr.2018.12.001
Goldim, Mariana Pereira ; Danielski, Lucineia Gainski ; Rodrigues, Judite Filgueiras ; Joaquim, Larissa ; Garbossa, Leandro ; de Oliveira Junior, Aloir Neri ; Metzker, Kiuanne Lino Lobo ; Giustina, Amanda Della ; Cardoso, Taise ; Barichello, Tatiana ; Petronilho, Fabricia. / Oxidative stress in the choroid plexus contributes to blood–cerebrospinal fluid barrier disruption during sepsis development. In: Microvascular Research. 2019 ; Vol. 123. pp. 19-24.
@article{d73db3a887b242c4995e9f42f999ec33,
title = "Oxidative stress in the choroid plexus contributes to blood–cerebrospinal fluid barrier disruption during sepsis development",
abstract = "Background: The choroid plexus (CP), main component of blood–cerebrospinal fluid barrier (BCSFB), protects the brain from peripheral inflammation similar to the blood–brain barrier. Thus, CP is considered a critical target site of oxidative damage, which in sepsis oxidative stress is likely to be a major step in the development of brain damage. Functional alterations in CP may be associated with sepsis-induced brain injury. However, there is no description on the mechanisms associated with BCSFB disruption during sepsis development. Materials and methods: To test this hypothesis, we examined time-dependent oxidative stress markers in CP and permeability of BCSFB in rats submitted to polymicrobial sepsis by cecal ligation and puncture (CLP) or sham surgery (control). We assessed albumin cerebrospinal fluid/plasma concentration quotient (Qalb), an index of BCSFB dysfunction and in CP samples, the oxidative damage in lipids, proteins, antioxidant enzymes and nitrite/nitrate (N/N) concentration in 12, 24 and 48 h after CLP. Results: The increase of BCSFB permeability is time-related to the increase of N/N concentration, oxidative damage to lipid and proteins, and decrease of antioxidant enzyme superoxide dismutase activity at 12 h in the CP; and decrease of catalase activity in 12 and 24 h. Conclusions: In experimental sepsis the BCSFB dysfunction occurs and oxidative stress seems to be a major step in this dysfunction.",
keywords = "Choroid plexus, Neuroinflammation, Oxidative stress, Sepsis",
author = "Goldim, {Mariana Pereira} and Danielski, {Lucineia Gainski} and Rodrigues, {Judite Filgueiras} and Larissa Joaquim and Leandro Garbossa and {de Oliveira Junior}, {Aloir Neri} and Metzker, {Kiuanne Lino Lobo} and Giustina, {Amanda Della} and Taise Cardoso and Tatiana Barichello and Fabricia Petronilho",
year = "2019",
month = "5",
day = "1",
doi = "10.1016/j.mvr.2018.12.001",
language = "English",
volume = "123",
pages = "19--24",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Oxidative stress in the choroid plexus contributes to blood–cerebrospinal fluid barrier disruption during sepsis development

AU - Goldim, Mariana Pereira

AU - Danielski, Lucineia Gainski

AU - Rodrigues, Judite Filgueiras

AU - Joaquim, Larissa

AU - Garbossa, Leandro

AU - de Oliveira Junior, Aloir Neri

AU - Metzker, Kiuanne Lino Lobo

AU - Giustina, Amanda Della

AU - Cardoso, Taise

AU - Barichello, Tatiana

AU - Petronilho, Fabricia

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Background: The choroid plexus (CP), main component of blood–cerebrospinal fluid barrier (BCSFB), protects the brain from peripheral inflammation similar to the blood–brain barrier. Thus, CP is considered a critical target site of oxidative damage, which in sepsis oxidative stress is likely to be a major step in the development of brain damage. Functional alterations in CP may be associated with sepsis-induced brain injury. However, there is no description on the mechanisms associated with BCSFB disruption during sepsis development. Materials and methods: To test this hypothesis, we examined time-dependent oxidative stress markers in CP and permeability of BCSFB in rats submitted to polymicrobial sepsis by cecal ligation and puncture (CLP) or sham surgery (control). We assessed albumin cerebrospinal fluid/plasma concentration quotient (Qalb), an index of BCSFB dysfunction and in CP samples, the oxidative damage in lipids, proteins, antioxidant enzymes and nitrite/nitrate (N/N) concentration in 12, 24 and 48 h after CLP. Results: The increase of BCSFB permeability is time-related to the increase of N/N concentration, oxidative damage to lipid and proteins, and decrease of antioxidant enzyme superoxide dismutase activity at 12 h in the CP; and decrease of catalase activity in 12 and 24 h. Conclusions: In experimental sepsis the BCSFB dysfunction occurs and oxidative stress seems to be a major step in this dysfunction.

AB - Background: The choroid plexus (CP), main component of blood–cerebrospinal fluid barrier (BCSFB), protects the brain from peripheral inflammation similar to the blood–brain barrier. Thus, CP is considered a critical target site of oxidative damage, which in sepsis oxidative stress is likely to be a major step in the development of brain damage. Functional alterations in CP may be associated with sepsis-induced brain injury. However, there is no description on the mechanisms associated with BCSFB disruption during sepsis development. Materials and methods: To test this hypothesis, we examined time-dependent oxidative stress markers in CP and permeability of BCSFB in rats submitted to polymicrobial sepsis by cecal ligation and puncture (CLP) or sham surgery (control). We assessed albumin cerebrospinal fluid/plasma concentration quotient (Qalb), an index of BCSFB dysfunction and in CP samples, the oxidative damage in lipids, proteins, antioxidant enzymes and nitrite/nitrate (N/N) concentration in 12, 24 and 48 h after CLP. Results: The increase of BCSFB permeability is time-related to the increase of N/N concentration, oxidative damage to lipid and proteins, and decrease of antioxidant enzyme superoxide dismutase activity at 12 h in the CP; and decrease of catalase activity in 12 and 24 h. Conclusions: In experimental sepsis the BCSFB dysfunction occurs and oxidative stress seems to be a major step in this dysfunction.

KW - Choroid plexus

KW - Neuroinflammation

KW - Oxidative stress

KW - Sepsis

UR - http://www.scopus.com/inward/record.url?scp=85058366500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058366500&partnerID=8YFLogxK

U2 - 10.1016/j.mvr.2018.12.001

DO - 10.1016/j.mvr.2018.12.001

M3 - Article

VL - 123

SP - 19

EP - 24

JO - Microvascular Research

T2 - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

ER -